Turkish Journal of Gastroenterology
Original Article

Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients

1.

Division of Pediatric Allergy and Immunology, Marmara University School of Medicine, Istanbul, Turkey

2.

Department of Gastroenterology, Marmara University School of Medicine, Istanbul, Turkey

3.

Department of Oral and Maxillofacial Surgery, Marmara University School of Medicine, Istanbul, Turkey

Turk J Gastroenterol 2020; 31: 400-409
DOI: 10.5152/tjg.2020.19358
Read: 199 Downloads: 69 Published: 02 May 2020

Background/Aims: Crohn’s Disease (CD) is a chronic inflammatory condition characterized by various abnormalities that lead to overly aggressive T-cell responses. Our in vitro experiments aimed to investigate the potential use of Dental Follicle Mesenchymal Stem Cells (DF-MSCs) to suppress the exaggerated immune response in inflamed and non-inflamed tissue of Crohn’s Disease (CD).

Material and Methods: Dental follicle tissues were obtained from extracted third molar teeth of 3 healthy volunteers who have no abscess or inflammatory diseases. Eleven patients included the experiment who had been diagnosed with CD and not received steroid maintenance therapy for more than 1 month. Mononuclear Cells (MNCs) were isolated from inflamed and non-inflamed tissue of CD. Isolated cells were stimulated with anti-CD3/anti-CD28 monoclonal antibodies in the presence and absence of DF-MSCs and analyzed for lymphocytes proliferation capacity and viability, T lymphocyte subsets, CD4+IL22BP and CD4+CD25+Foxp3+ regulatory T cell (Tregs) frequencies and cytokine levels.

Results: A significant downregulation of lymphocyte proliferation and CD4+IL22BP T cell ratio were found in inflamed cultures with DF-MSCs (p<0,005). Also, the frequency of Tregs increased with DF-MSCs (p<0,05). Pro-inflammatory cytokine levels (TNF-α and IL-6) were decreased (p<0,05) and IL-10 levels were increased (p<0,05) in the supernatant of inflamed cultures.

Conclusion: DF-MSCs reduced the inflammatory immune response, induced Tregs and downregulated CD4+IL22BP T cell ratio in inflamed samples of CD patients, which may be exploited for significant therapeutic use.

Cite this article as: Zibandeh N, Genç D, Duran Y, et al. Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. Turk J Gastroenterol 2020; 5: 400-9.

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