Turkish Journal of Gastroenterology
Liver - Original Article

Diagnosis of cirrhosis in patients with chronic hepatitis C genotype 4: Role of ABCB11 genotype polymorphism and plasma bile acid levels

1.

Department of Tropical Medicine, Mansoura University, Mansoura, Egypt

2.

Department of Tropical Medicine, Mansoura University, Mansoura,

3.

Department of Pathology, Mansoura University, Mansoura, Egypt

4.

Department of Clinical Pathology, Mansoura University, Mansoura, Egypt

5.

Department of Diagnostic Radiology, Mansoura University, Mansoura, Egypt

Turk J Gastroenterol 2018; 29: 299-307
DOI: 10.5152/tjg.2018.17570
Read: 1803 Downloads: 674 Published: 25 July 2019

Abstract

 

Background/Aims: Chronic hepatitis C (CHC)-related mortality generally results from cirrhosis and subsequent complications. We aimed to investigate the potential role of plasma bile acid levels and ABCB11 1331T>C (V444A, rs2287622) (ATP-binding cassette subfamily B, member 11) gene polymorphism in fibrosis prediction in CHC genotype 4 patients.

 

Materials and Methods: This case control study included 85 healthy control and the following 225 subjects: 170 adult patients infected with hepatitis C virus (HCV) and categorized into three groups according to liver biopsy; no fibrosis group (F0) (n=33), early fibrosis group (F1-F2) (n=61), and advanced fibrosis group (F3-F4) (n=76). Fasting bile acid levels, hepatitis C virus (HCV) genotyping, and ABCB11 1331T>C gene polymorphism were assessed.

 

Results: The frequency of the variant homozygote genotype CC in advanced fibrosis was significantly higher than that in early fibrosis (48.7% vs. 36.1%) (odd ratio, OR =2.58; 95% confidence interval, CI=1.07-6.20; p=0.03). C allele was significantly represented in advanced fibrosis (65.8%) compared with that in early fibrosis (51.6%) (OR=1.80, 95% CI=1.10-2.93, p=0.01). A significant elevation of plasma bile acid levels in advanced fibrosis was observed compared with those in early fibrosis (p≤0.001). Receiver operating characteristic curve for plasma bile acid levels at cutoff value of 75.5 μmol/L had a 59% specificity and 97.4% sensitivity as a predictor of advanced hepatic fibrosis (AUROC=0.78%).

 

Conclusion: We concluded that Egyptian patients having chronic hepatitis C genotype 4 with CC genotype of ABCB11 SNP 1331T>C and high plasma bile acid levels at cutoff value of 75.5 μmol/L were associated with advanced hepatic fibrosis.

 

Cite this article as: Besheer T, Arafa M, Abd El-Maksoud Mohamed, et al. Diagnosis of cirrhosis in patients with chronic hepatitis C genotype 4: Role of ABCB11 genotype polymorphism and plasma bile acid levels. Turk J Gastroenterol 2018; 29: 299-307

Files
EISSN 2148-5607