Published In
Turkish Journal of Gastroenterology
Volume 36 · Issue 10
Pages: 658-668
History
Published Online: May 5, 2025
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright © 2025 Ke Chen, Jia-Wei Chen, Yao Shen, Yun-Fei Wang, Sheng-Rong Dong, Xiao-Xue Zhang, Chen-Yang Li, Xiao-Juan Gao, Jia-Min Zhao, Yu-Nan Zhang, Wen-Ying Tian, Jia-Le Lv, Qiang Zhan, Fang-Mei An
Ke Chen
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Jia-Wei Chen
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Yao Shen
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Yun-Fei Wang
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Sheng-Rong Dong
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Xiao-Xue Zhang
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Chen-Yang Li
Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China
Xiao-Juan Gao
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Jia-Min Zhao
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Yu-Nan Zhang
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Wen-Ying Tian
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Jia-Le Lv
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Qiang Zhan
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Fang-Mei An
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Chen, K., Chen, J.-W., Shen, Y., Wang, Y.-F., Dong, S.-R., Zhang, X.-X., … An, F.-M. (2025). Helicobacter pylori Promoted miR-196a/b-5p Expression and Accelerated Tumorigenesis of the Gastric Mucosa by Targeting IGF2BP1 and Activating PI3K-Akt Signaling Pathway. Turkish Journal of Gastroenterology, 36(10), 658–668. https://doi.org/10.5152/tjg.2025.24397
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Abstract
Background/Aims: The study focuses on examining the impact of Helicobacter pylori (H. pylori) on the modulation of the miRNA expres sion profiles while also unraveling the associated pathways that play a significant role in initiating and driving the development of gastric cancer (GC).
Materials and Methods: An in-depth analysis of miRNA expression profiles in gastric tissue samples from patients with chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG), dysplasia (Dys), or GC was conducted. The carbon-13 urea breath test was used to identify H. pylori infection, and the participant cohort was characterized by the presence of H. pylori infection. Additionally, the role of miR-196a/b-5p in GC carcinogenesis was investigated.
Results: A total of five miRNAs—miR-196a-5p, 196b-5p, 224-5p, 424-3p, and 941—demonstrated marked elevation in CSG, CAG, Dys, and GC. miR-196a/b-5p was observed to be upregulated in GC cells following H. pylori infection, as well as in Dys and GC tissue samples from patients harboring H. pylori. miR-196a/b-5p can expedite GC progression. Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), the target gene of miR-196a/b-5p, diminishes the proliferation capability of GC cells; however, miR-196a/b-5p can partially counteract this effect. miR-196a/b-5p activates the PI3K-Akt pathway, while IGF2BP1 inhibits the expression of these proteins.
Conclusion: The levels of miR-196a/b-5p were observed to escalate following H. pylori infection, subsequently fostering the progression of GC by specifically targeting IGF2BP1 and triggering the PI3K-Akt signaling cascade.
Cite this article as: Chen K, Chen JW, Shen Y, et al. Helicobacter pylori promoted miR 196a/b-5p expression and accelerated tumorigenesis of the gastric mucosa by targeting IGF2BP1 and activating PI3K-Akt signaling pathway. Turk J Gastroenterol. 2025;36(10):658-668.
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Article Info
Published In
Journal
Turkish Journal of Gastroenterology
Volume / Issue
Volume 36 · Issue 10
Pages
658-668
History
Published Online
May 5, 2025
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright
Copyright (c) 2025 Ke Chen, Jia-Wei Chen, Yao Shen, Yun-Fei Wang, Sheng-Rong Dong, Xiao-Xue Zhang, Chen-Yang Li, Xiao-Juan Gao, Jia-Min Zhao, Yu-Nan Zhang, Wen-Ying Tian, Jia-Le Lv, Qiang Zhan, Fang-Mei An
Affiliations
Ke Chen
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Jia-Wei Chen
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Yao Shen
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Yun-Fei Wang
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Sheng-Rong Dong
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Xiao-Xue Zhang
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Chen-Yang Li
Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China
Xiao-Juan Gao
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Jia-Min Zhao
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Yu-Nan Zhang
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Wen-Ying Tian
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Jia-Le Lv
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Qiang Zhan
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Fang-Mei An
Department of Gastroenterology, Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Jiangsu, China
Cite this Article
Chen, K., Chen, J.-W., Shen, Y., Wang, Y.-F., Dong, S.-R., Zhang, X.-X., … An, F.-M. (2025). Helicobacter pylori Promoted miR-196a/b-5p Expression and Accelerated Tumorigenesis of the Gastric Mucosa by Targeting IGF2BP1 and Activating PI3K-Akt Signaling Pathway. Turkish Journal of Gastroenterology, 36(10), 658–668. https://doi.org/10.5152/tjg.2025.24397
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