Role of Th1/Th2 cells and related cytokines in autoimmune hepatitis
Clinical Genetics Department, National Institute of Genetic Engineering and Biotechnology Tehran, Iran
Digestive Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Turk J Gastroenterol 2017; 28: 110-114
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Key Words: Autoimmune hepatitis, gene expression, cytokines
Background/Aims: Dysregulation of T cell response is thought to play an important role in the immunopathogenesis of autoimmune hepatitis. However, no consensus has yet been reached regarding the implications of a distinct T cell subset in the pathogenesis of this progressive liver disease. Therefore, T-bet and GATA-3 expression was examined in patients with autoimmune hepatitis (AIH) and in healthy controls. Moreover, the profile of Th1 (IFN-γ) and Th2 (IL-4) cytokine gene expression was analyzed.
Materials and Methods: Levels of mRNA transcripts were measured in peripheral blood mononuclear cells (PBMCs) using a two-step reverse transcription quantitative real-time polymerase chain reaction with SYBR Green.
Results: T-bet and IFN-γ mRNA expression was significantly higher in AIH patients compared to healthy controls (p<0.05), whereas no differences were observed for either GATA-3 or IL-4 mRNA expression (p>0.05).
Conclusion: Alterations in the Th1/Th2 cell balance may be responsible for both disease progression and the resulting complications.