ISSN 1300-4948 | E-ISSN 2148-5607
Original Article
Influences of proton pump inhibitor on Helicobacter pylori adherence to the gastrointestinal cell lines
1 Faculty of Pharmacy, University Kebangsaan Malaysia, Kuala Lumpur, Malaysia  
Turk J Gastroenterol 2017; 28: 53-59
DOI: 10.5152/tjg.2016.0409
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Key Words: Proton pump inhibitor, protein expression, H. pylori

Background/Aims: Helicobacter pylori is a carcinogenic bacterium that could induce P-glycoprotein expression in the human gastrointestinal tract. Bacterial adherence to the gastrointestinal cell lines could be influenced by the level of P-glycoprotein. This study aimed to determine the influence of proton pump inhibitors that exhibit an inhibitory effect on P-glycoprotein in gastrointestinal carcinoma cell lines, namely Caco-2 and LS174T, in relation to H. pylori adherence.


Materials and Methods: Caco-2 and LS174T cells lines treated with omeprazole and esomeprazole were used in this study to assess the bacterial attachment of H. pylori within certain incubation periods.


Results: The presence of proton pump inhibitors increased the H. pylori adherence in a time-dependent manner in both Caco-2 and LS174T cell lines. The double inhibition of P-glycoprotein using proton pump inhibitor and P-glycoprotein inhibitor caused low P-glycoprotein expression in the cell lines, resulting in higher H. pylori adherence compared to the control cell lines.


Conclusion: Proton pump inhibitors may alter P-glycoprotein expression in the gastrointestinal tract, and subsequently H. pylori adherence on the cell lines, and may contribute to resistance to drug therapy.

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